This book deals with the seperation of Glutamic oxalic acetate transaminase isoenzymes and some of their physical and chemical properties in sera of patients with obstructive jaundice and Myocardial Infarction . The fractionation of GOT isoenzymes in sera of patients with obstructive and Myocardial infarction and then study the spectral studies , electrophoretic pattern , molecular weight determination and isoelectric points determination.