The polymerase chain reaction (PCR) has proved to be a powerful and versatile tool and has opened new avenues in molecular biol- ogy. Alternative nucleic acid amplification techniques, such as the ligase chain reaction (LCR), nucleic acid sequence-based amplifica- tion (NASBA), and transcription-mediated amplification (TMA), a variation of NASBA, are also now available. These techniques are all designed to amplify specific nucleic acid sequences in...
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