Part I: High-Throughput Cloning, Expression Screening, and Optimization 1. Recombinant Protein Expression in E. coli: A Historical Perspective Opher Gileadi 2. N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools Christopher D.O. Cooper and Brian D. Marsden